CLEAN TEST is a rapid swab test designed for the hygienic control of work surfaces. It detects residues of proteins, fats, and sugars, indicating the cleanliness level of a particular surface. This test is essential in environments where hygiene is paramount, such as food processing facilities, restaurants, and medical settings.
Composition and Principle
The CLEAN TEST kit comprises plastic tubes containing a swab, a vial (reagent A), and a disc (reagent B). The test is based on the reaction of bicinchoninic acid (reagent A) with cupric sulfate (reagent B). In alkaline conditions, this reaction leads to the complexation of Cu ions with the peptide bonds of proteins, resulting in a purple coloration directly proportional to the concentration of proteins, fats, and sugars on the surface.
The testing procedure involves wetting the swab with physiological solution or sterile distilled water, then streaking it across a 10 x 10 cm area of the surface. After swabbing, the swab is returned to the tube, the glass vial is broken to release reagent A, and the tube is vigorously shaken. After 8-10 minutes, the color of the solution is compared to a colorimetric scale to determine the cleanliness level.
Interpretation of Results
The color developed in the test is indicative of surface cleanliness:
– A pale green, colorless, or clear light grey indicates proper cleaning.
– Dark grey suggests the need for rinsing or retesting.
– Light or dark purple signifies the necessity of a new cleaning cycle with suitable detergents.
False positives may occur in the presence of peroxides, ascorbic acid, or other strongly reducing agents, while false negatives might result from high concentrations of alkaline detergents.
Quality Control for the User
Quality control includes checking general characteristics, labeling, and printing, as well as chemical control. The product’s efficiency is verified using standard protein solutions, with different colorations indicating varying concentrations of protein residues.
Warnings and Precautions
Though not classified as hazardous, it is recommended to consult the safety data sheet for handling. The test should be performed in environments with temperatures ranging from 10 to 25°C and should not be conducted in temperatures below 0°C.
CLEAN TEST should be stored at temperatures between 10-30°C, away from light, and used before the expiry date on the label. Any signs of deterioration or contamination necessitate the disposal of the product.
Disposal of Used Material
Used material should be disposed of according to national and local regulations.
The CLEAN TEST is a crucial tool in maintaining high standards of hygiene on work surfaces, especially in sensitive environments. Its ease of use, coupled with its reliable and clear results, makes it an indispensable asset for ensuring cleanliness and safety in various settings.