**Introduction**
Novobiocin MKTT Supplement is a selective supplement specifically designed for the detection of Salmonella spp. This product is used in conjunction with Mueller Kauffman Broth Base for the enrichment of testing media. It is an essential tool in microbiological laboratories, especially in food safety testing and environmental monitoring.
**Composition and Principle**
The key component of Novobiocin MKTT Supplement is novobiocin, an antibiotic effective against both Gram-positive and some Gram-negative bacteria. Each kit contains 10 vials of the freeze-dried supplement, with each vial containing 20.0 mg of novobiocin, translating to 40.0 mg per liter of prepared medium. The presence of novobiocin in the medium provides selective pressure against non-target bacteria, thereby enhancing the detection and growth of Salmonella spp.
**Procedure for Use**
To use the Novobiocin MKTT Supplement, one must first reconstitute the contents of a vial with 5 ml of sterile distilled water. The reconstituted supplement is then added to 500 ml of boiled and cooled Mueller Kauffman Broth Base, which has been supplemented with Iodine MKTT Solution. Careful mixing and subsequent distribution into sterile tubes are necessary to ensure homogeneity. The prepared medium should then be used following standard microbiological practices for the detection of Salmonella spp.
**Quality Control and Interpretation of Results**
Quality control of the Novobiocin MKTT Supplement involves both visual and microbiological assessments. Visually, the freeze-dried product should appear white. Microbiologically, the effectiveness of the supplement is confirmed by inoculating the prepared medium with specific control strains, including Salmonella typhimurium and Escherichia coli. Salmonella typhimurium should exhibit good growth, while growth of E. coli should be inhibited. These tests confirm the selective nature of the supplement and its suitability for detecting Salmonella spp.
**Storage and Precautions**
Novobiocin MKTT Supplement should be stored at 2-8°C in its original packaging, away from heat sources and excessive temperature fluctuations. It remains effective until the expiry date on the label, provided storage conditions are met. As the supplement is classified as hazardous under current legislation, it is recommended that laboratories follow the safety data sheet guidelines for handling. Only trained personnel should handle this supplement, using appropriate aseptic techniques and safety measures.
**Conclusion**
In summary, Novobiocin MKTT Supplement is a vital component in the detection of Salmonella spp., especially in food safety and environmental testing. Its selective action against non-target bacteria and its compatibility with Mueller Kauffman Broth Base make it a reliable and essential tool in microbiological laboratories.
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